Methylene Blue
About
About Methylene Blue
Methylene blue is a century-old compound originally used as an antimalarial and antidote for methemoglobinemia, now studied for cognitive enhancement, mitochondrial support, and neuroprotection. At low doses it acts as a mitochondrial electron carrier, improving cellular energy production. It has shown promise in early Alzheimer's research and is used off-label by biohackers for focus and memory. Pharmaceutical-grade formulations are essential for safety.
Science
Mechanism of Action
At low doses, functions as a mitochondrial electron shuttle between NADH and cytochrome c, bypassing complex I/III dysfunction and increasing ATP production; also inhibits MAO and nitric oxide synthase at higher doses.
Dosing
Typical Protocol
0.5–4 mg/kg oral or IV; low-dose protocol: 5–15 mg oral 2–3x per week. Avoid high doses and SSRI interactions.
⚠ Protocol information is for educational purposes only. Dosing must be determined by a licensed physician based on individual health status and goals.
Regulatory
Legal Status in 2026
This compound is legal to prescribe and dispense through a licensed 503A or 503B compounding pharmacy with a valid prescription. It is not FDA-approved as a finished drug product, but compounding is explicitly permitted under federal law. Quality and sterility standards vary by pharmacy — look for PCAB-accredited compounders.
Evidence
Evidence Tier
Evidence primarily from animal studies, cell cultures, or small pilot human trials. Results are promising but have not yet been confirmed in large-scale human RCTs. Effects in humans may differ from animal models.
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